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Stains, Fluorescent Dyes & Histology Reagents

Methylene blue

MicroscopyAvailable on request

Order Methylene blue (CAS 122965-43-9) online at Zemixal. Lab and bulk packs, batch COA, fast global delivery. Catalogue no. ZX-000631.

Pack sizes
Methylene blue chemical structure
CAS number122965-43-9
FormulaC16H20ClN3OS
Molar mass337.9 g/mol
Physical formPowder
HS code32041351
InChIKeyWQVSELLRAGBDLX-UHFFFAOYSA-M
IUPAC name[7-(dimethylamino)phenothiazin-3-ylidene]-dimethylazanium;chloride;hydrate

About Methylene blue

Researchers and plant chemists source Methylene blue from Zemixal for routine and specialised work. Identity at a glance (Microscopy): CAS 122965-43-9, formula C16H20ClN3OS and molar mass 337.9 g/mol. Expect dark green to very dark green powder.

Typical applications

  • Histology & cytology staining
  • Microscopy
  • Clinical laboratories
  • Biological staining

Safe handling

It carries the GHS signal word Warning (H302), so wear gloves and eye protection and work with good ventilation.

Available as 25 g, 100 g, 1 kg and 25 kg. Larger or split packs can be arranged. Each lot travels with a batch certificate of analysis and a 16-section SDS. Add it to your quote list and our team replies with price, lead time and shipping options within one business day.

Typical uses

  • Histology & cytology staining
  • Microscopy
  • Clinical laboratories
  • Biological staining

Applications

Specifications and identifiers

Specifications of Methylene blue
Product nameMethylene blue
Catalogue numberZX-000631
CAS number122965-43-9
IUPAC name[7-(dimethylamino)phenothiazin-3-ylidene]-dimethylazanium;chloride;hydrate
Molecular formulaC16H20ClN3OS
Molar mass337.9 g/mol
Exact mass337.1016
GradeMicroscopy
Physical formPowder
AppearanceDark green to very dark green powder
H-bond donors / acceptors1 / 5
Topological polar surface area44.9 Ų
HS code32041351
InChIKeyWQVSELLRAGBDLX-UHFFFAOYSA-M
SMILESCN(C)C1=CC2=C(C=C1)N=C3C=CC(=[N+](C)C)C=C3S2.O.[Cl-]
PubChem CID16211647
Synonyms3,7-bis(Dimethylamino)phenazathionium chloride, Basic Blue 9, Tetramethylthionine chloride, Methylene Blue hydrate, Methylene Blue monohydrate, Basic Blue 9 monohydrate, Methylene Blue (hydrate), C.i. basic Blue 9 monohydrate, 3,7-Bis(dimethylamino)phenothiazin-5-ium chloride hydrate(1:1:x), 3,7-Bis(dimethylamino)phenothiazin-5-ium chloride xhydrate, Phenothiazin-5-ium, 3,7-bis(dimethylamino)-, chloride, hydrate (1:1:3)

Lot values are stated on the batch certificate of analysis. Ask for a typical COA with your quotation.

Safety and GHS hazards

GHS07 Irritant or harmful
Irritant or harmful

Signal word Warning

  • H302Harmful if swallowed

Precautionary statements P264, P270, P301 + P317, P330, P501

Classification summarised from public GHS notifications. It can vary with grade and concentration, so always follow the SDS supplied with your lot.

Pack sizes

Pack sizes of Methylene blue
25 gQuoted on request
100 gQuoted on request
1 kgQuoted on request
25 kgQuoted on request

Need a different size? Bulk drums and custom packing are quoted on request through the quote form on this page.

Questions about Methylene blue

Which CAS number identifies Methylene blue?
Methylene blue is registered under CAS 122965-43-9. You may also see it listed as 3,7-bis(Dimethylamino)phenazathionium chloride, Basic Blue 9, Tetramethylthionine chloride. Our catalogue number for this item is ZX-000631.
What are the formula and molar mass of Methylene blue?
For Methylene blue, its formula is C16H20ClN3OS and its molar mass is 337.9 g/mol. InChIKey WQVSELLRAGBDLX-UHFFFAOYSA-M lets you match it in any structure database.
What grade and pack sizes are available for Methylene blue?
This listing is Microscopy grade. Standard packs are 25 g, 100 g, 1 kg, 25 kg. Larger quantities and custom packing can be quoted on request.
Which documents come with Methylene blue?
Each lot ships with a batch certificate of analysis and a 16-section GHS safety data sheet. A typical COA or specification sheet can be shared with the quotation before you order.
Can Zemixal ship Methylene blue to my country?
Yes. Because Methylene blue is classed as hazardous, it travels in UN-approved packaging as dangerous goods under IATA or IMDG rules, with the export paperwork your broker needs.

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