Methotrexate

Name: Methotrexate
Brand: Zemixal
SKU: ZX-006646

Reference StandardAvailable on request

Order Methotrexate (CAS 59-05-2) online at Zemixal: C20H22N8O5, COA and SDS with every lot, worldwide shipping. Part of our API Reference Standards range.

Pack sizes

CAS number59-05-2

FormulaC₂₀H₂₂N₈O₅

Molar mass454.4 g/mol

Physical formSolid

HS code38229090

InChIKeyFBOZXECLQNJBKD-ZDUSSCGKSA-N

IUPAC name(2S)-2-[[4-[(2,4-diaminopteridin-6-yl)methyl-methylamino]benzoyl]amino]pentanedioic acid

About Methotrexate

Analytical teams working on Methotrexate use Methotrexate as a qualified comparison standard. For your records (Reference Standard), the material is defined by CAS 59-05-2, formula C20H22N8O5 and molar mass 454.4 g/mol. It is handled as a solid.

Typical applications

Identification & assay
Method development & validation
QC reference material

Safe handling

Hazard communication follows GHS (Danger: H301, H315, H319 and H340). Our SDS lists exposure controls and first-aid measures.

Standard pack sizes are 10 mg, 25 mg, 50 mg and 100 mg, and custom quantities are quoted on request. Every shipment includes a certificate of analysis with characterisation data. Send an enquiry through the quote panel and you get a formal offer with Incoterms and delivery time.

Reference standards for Methotrexate

See every impurity, metabolite and related compound we list for this API.

Methotrexate standards

Typical uses

Identification & assay
Method development & validation
QC reference material

Applications

Pharmaceutical Manufacturing and QC Research Labs and Universities

Specifications and identifiers

Specifications of Methotrexate
Product name	Methotrexate
Catalogue number	ZX-006646
CAS number	59-05-2
IUPAC name	(2S)-2-[[4-[(2,4-diaminopteridin-6-yl)methyl-methylamino]benzoyl]amino]pentanedioic acid
Molecular formula	C₂₀H₂₂N₈O₅
Molar mass	454.4 g/mol
Exact mass	454.1713
Grade	Reference Standard
Physical form	Solid
XLogP3	-1.8
H-bond donors / acceptors	5 / 12
Topological polar surface area	211 Å²
HS code	38229090
InChIKey	FBOZXECLQNJBKD-ZDUSSCGKSA-N
SMILES	CN(CC1=CN=C2C(=N1)C(=NC(=N2)N)N)C3=CC=C(C=C3)C(=O)N[C@@H](CCC(=O)O)C(=O)O
PubChem CID	126941
Synonyms	N-[4-[[(2,4-Diamino-6-pteridinyl)methyl]methylamino]benzoyl]-L-glutamic acid, (+)-Amethopterin, (2S)-2-[[4-[[(2,4-Diaminopteridin-6-yl)methyl]methylamino]benzoyl]amino]pentanedioic acid, 4-Amino-10-methylfolic acid, 4-Amino-N10-methylfolic Acid, Antifolan, Emtexate, Ledertrexate, MTX, L-Metatrexan, Methylaminopterin, Mexate

Lot values are stated on the batch certificate of analysis. Ask for a typical COA with your quotation.

Safety and GHS hazards

Acute toxicity

Irritant or harmful

Health hazard

Signal word Danger

H301Toxic if swallowed
H315Causes skin irritation
H319Causes serious eye irritation
H340May cause genetic defects
H360May damage fertility or the unborn child

Precautionary statements P203, P264, P264 + P265, P270, P280, P301 + P316, P302 + P352, P305 + P351 + P338, P318, P321, P330, P332 + P317, P337 + P317, P362 + P364, P405, P501

Classification summarised from public GHS notifications. It can vary with grade and concentration, so always follow the SDS supplied with your lot.

Pack sizes

Pack sizes of Methotrexate
10 mg	Quoted on request
25 mg	Quoted on request
50 mg	Quoted on request
100 mg	Quoted on request

Need a different size? Bulk drums and custom packing are quoted on request through the quote form on this page.

Questions about Methotrexate

Which CAS number identifies Methotrexate?

Methotrexate is registered under CAS 59-05-2. You may also see it listed as N-[4-[[(2,4-Diamino-6-pteridinyl)methyl]methylamino]benzoyl]-L-glutamic acid, (+)-Amethopterin, (2S)-2-[[4-[[(2,4-Diaminopteridin-6-yl)methyl]methylamino]benzoyl]amino]pentanedioic acid. Our catalogue number for this item is ZX-006646.

What are the formula and molar mass of Methotrexate?

For Methotrexate, its formula is C20H22N8O5 and its molar mass is 454.4 g/mol. The systematic (IUPAC) name is (2S)-2-[[4-[(2,4-diaminopteridin-6-yl)methyl-methylamino]benzoyl]amino]pentanedioic acid. InChIKey FBOZXECLQNJBKD-ZDUSSCGKSA-N lets you match it in any structure database.

How is Methotrexate used in pharmaceutical QC?

Methotrexate is a characterised reference material linked to Methotrexate. Laboratories use it to identify and quantify this impurity during method development and validation, stability work, batch release testing and regulatory submissions.

What grade and pack sizes are available for Methotrexate?

This listing is Reference Standard grade. Standard packs are 10 mg, 25 mg, 50 mg, 100 mg. Larger quantities and custom packing can be quoted on request.

Which documents come with Methotrexate?

Each lot ships with a batch certificate of analysis and a 16-section GHS safety data sheet. A typical COA or specification sheet can be shared with the quotation before you order.

Can Zemixal ship Methotrexate to my country?

Yes. Because Methotrexate is classed as hazardous, it travels in UN-approved packaging as dangerous goods under IATA or IMDG rules, with the export paperwork your broker needs.

Other grades with CAS 59-05-2

Methotrexate

CAS: 59-05-2
Formula: C₂₀H₂₂N₈O₅
MW: 454.4 g/mol

ZX-004219

Methotrexate, 98%

CAS: 59-05-2
Formula: C₂₀H₂₂N₈O₅
MW: 454.4 g/mol

ZX-011038

More Methotrexate standards

Have a list of compounds to price?

Paste up to 200 CAS numbers into Bulk CAS lookup, or send one enquiry for everything in your quote list. We reply with price, lead time and documents.

Bulk CAS lookup Open quote list