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Solvents (HPLC & GC)

Spectroscopy, NMR & Specialty Grade Solvents

Spectroscopy, deuterated NMR, scintillation, electronic and DNA synthesis grade solvents with tight limits for optical and trace-sensitive work.
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Some techniques are more demanding than chromatography. UV-Vis and fluorescence spectroscopy need solvents that are transparent at short wavelengths. NMR uses deuterated solvents to remove the solvent signal. Oligonucleotide synthesis needs very dry acetonitrile, and electronics manufacturing needs very low metal content. This sub-category groups those specialist grades, such as Acetonitrile for DNA 99.5%, Acetonitrile-D3 99.8%, 1,4-Dioxan scintillation grade and Acetone electronic grade 99.5%+.

For each grade, check the figure that matters for your technique. That means transmittance at the cut-off for spectroscopy, isotopic enrichment and water for NMR, water in ppm for DNA synthesis, and metal ions in ppb for electronics. Our listings state the grade, and the COA reports the measured values.

Deuterated solvents come in sealed ampoules or septum bottles, and dry grades are packed under inert gas. Store them tightly closed and away from moisture. Express shipping is available for small packs.

Spectroscopy, NMR & Specialty Grade Solvents: questions buyers ask

What does 99.8% D mean on a deuterated solvent?
It is the isotopic enrichment, meaning 99.8% of the hydrogen positions carry deuterium. The remaining 0.2% hydrogen gives the small residual solvent peak used as a chemical shift reference. Higher enrichment, such as 99.96%, gives a smaller residual peak, which helps when analyte signals fall near the solvent signal.
How dry must acetonitrile be for oligonucleotide synthesis?
Phosphoramidite coupling is very sensitive to water, so acetonitrile for DNA synthesis usually carries a limit in the low tens of ppm. Higher water levels reduce coupling efficiency and full-length yield. Keep bottles sealed under inert gas, use dry transfer lines, and avoid leaving the solvent open on the synthesiser.
Why is electronic grade acetone priced above reagent grade?
Electronic grade solvents are purified and packed to keep metal ions, anions and particles at very low levels, often in parts per billion. These contaminants would damage semiconductor wafers or thin films. The extra purification, clean packaging and trace analysis add cost compared with reagent grades.

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