Molecular Biology Grade chemicals

What the grade means

In molecular biology a reagent can be chemically pure and still ruin an experiment. A trace of RNase degrades RNA, metal contaminants inhibit polymerases and protease activity damages enzymes. Molecular biology grade combines a normal purity specification with functional tests for these hazards.

Typical specification points

• Assay of at least 99 percent, often 99.5 percent.
• DNase, RNase and protease activity not detected.
• Low ppm limits for heavy metals or named metals.
• Clear, colourless solution at a stated concentration.
• Low UV absorbance at 260 and 280 nm where relevant.

When to choose it

Use this grade for PCR and qPCR buffers, DNA and RNA extraction, electrophoresis, sequencing workflows and enzyme reactions. Typical products are Tris, EDTA, magnesium chloride, guanidine salts, SDS, agarose and sucrose. For general biochemistry or buffer preparation outside nucleic acid work, a standard biochemical grade is usually adequate.

Documentation

Each lot ships with a COA reporting the enzyme activity tests and a GHS SDS. Browse Biochemicals for products.