HPLC Grade chemicals

What the grade guarantees

An HPLC grade solvent is judged by what it adds to your chromatogram, not only by its assay. Trace aromatics raise UV background, stabilisers create ghost peaks in gradients, non-volatile residue builds up on the column head and particles block frits. The grade controls each of these.

Typical specification points

• Assay by GC of 99.8 to 99.9 percent or higher.
• Absorbance limits at several wavelengths, for example acetonitrile at or below 0.02 AU at 200 nm.
• Water by Karl Fischer, usually 0.02 to 0.05 percent maximum.
• Residue on evaporation of a few mg/L at most.
• Filtration through a 0.2 µm membrane.

When to choose it

Use HPLC grade for UV and diode-array methods, sample preparation for those methods and dissolution testing. If you run steep gradients at low wavelength, ask for gradient grade, which is tested with a blank gradient. For mass spectrometry, step up to LC-MS grade.

Documentation

Each lot ships with a COA reporting the tested values, plus a 16-section GHS SDS. Browse the Solvents (HPLC and GC) division for available products.