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Biochemicals

Cell Culture Reagents

Media supplements, cyclodextrins, nucleotides, polyphenols and lipids tested or suited for mammalian and microbial cell culture work.
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Cells respond to everything in their medium, so supplements need to be pure, consistent and free of contaminants that stress growth. This sub-category offers additives and reagents used in culture work, from solubilisers and nutrients to signalling compounds. Examples include (2-Hydroxypropyl)-β-cyclodextrin for cell culture 99%+, (±)-Carnitine hydrochloride, (−)-Epicatechin at ≥98% by HPLC and 2′-Deoxyadenosine 5′-monophosphate.

Ask three questions before buying. Is the material tested for cell culture or endotoxin? Is the solubility right for your stock concentration? Will it remain stable at 37 °C over the culture period? Cyclodextrins, for example, are used to deliver lipophilic compounds or remove cholesterol from membranes, and their degree of substitution affects both uses.

Products are packed in sealed vials and bottles and shipped cold where needed. Lot COAs report purity and identity, with additional testing stated where it was done. If you are scaling a process, ask about kilogram packs from a single lot.

Cell Culture Reagents: questions buyers ask

Why is hydroxypropyl-β-cyclodextrin used in cell culture?
It forms inclusion complexes with poorly water-soluble molecules such as cholesterol, steroids and lipophilic drugs, carrying them into aqueous media. It is also used to remove cholesterol from cell membranes in signalling studies. Its higher solubility and lower toxicity make it preferable to native beta-cyclodextrin for most cell work.
How do I sterilise supplements before adding them to media?
Most small-molecule supplements are dissolved in water or buffer and passed through a 0.22 µm sterile filter. Avoid autoclaving heat-sensitive compounds such as nucleotides, vitamins and polyphenols. Prepare concentrated stocks, filter them, and store aliquots frozen to limit repeated handling.
What does cell culture tested mean on a product?
It means a representative lot was checked for its effect on the growth of a reference cell line and, usually, for endotoxin or sterility. The exact tests vary by manufacturer and grade. Look at the COA for the tests reported, and validate the material in your own cell system before routine use.

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