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Biochemicals

Enzymes, Proteins & Inhibitors

Chromogenic and fluorogenic enzyme substrates, enzyme inhibitors and protein-chemistry reagents for assays, histochemistry and biochemical research.
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Measuring an enzyme usually means giving it a substrate that changes colour or fluorescence when it reacts. This sub-category gathers such substrates together with inhibitors and protein-related reagents. Esterase substrates like 1-Naphthyl acetate GR 99%+, 2-Naphthyl acetate and 1-Naphthyl butyrate feature alongside 2,7-Dichlorofluorescein in ACS reagent grade and 1-Methylimidazole, a catalyst and buffer component.

For substrates, look at purity and the level of free chromophore, since free naphthol or fluorescein raises background before the enzyme acts. For inhibitors, check potency data, solubility and stability in your assay buffer. Many naphthyl esters are used in histochemical staining of blood cells and tissues as well as in plate assays.

Products are packed in small sealed vials or bottles, many protected from light. Store them as the label directs, often cold and dry. Each lot comes with a COA, and further assay information can be requested through the quote panel.

Enzymes, Proteins & Inhibitors: questions buyers ask

Why does free naphthol content matter in esterase substrates?
Esterase assays detect the naphthol released when the enzyme cleaves the ester. Any naphthol already present in the substrate produces signal without enzyme activity, raising the blank. Low free naphthol gives a cleaner baseline and better sensitivity, especially in kinetic assays and histochemical staining.
How should substrate stock solutions be prepared?
Dissolve the substrate in a suitable organic solvent such as DMSO or acetone at high concentration, then dilute into assay buffer just before use. Many esters hydrolyse slowly in water, so aqueous working solutions should be fresh. Store stocks frozen in small aliquots to avoid repeated thawing.
Can these reagents be used in clinical diagnostics?
They are supplied as research reagents. Several are used in diagnostic staining protocols, but clinical use may require registered diagnostic products or validated grades depending on your country. Confirm your regulatory needs, and use ready-made diagnostic solutions where your laboratory accreditation requires them.

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